Clinical Research Evidence

Dynamic Light's SpeckleView® technology has 20 years of research evidence to support its utility in the operating room. Back in 2001, co-founder Andrew Dunn demonstrated the first use of laser speckle to image blood flow in a rodent brain. Andrew Dunn translated the use of laser speckle to the operating room and published the seminal paper in 2010 demonstrating the ability of SpeckleView technology to monitor cerebral blood flow during neurosurgery. This was followed with a demonstration of SpeckleView technology with an endoscope in 2013. A 2014 paper showed the utility of SpeckleView technology in 10 neurosurgery patients, followed by a 2017 demonstration of quantitative blood flow monitoring in 8 neurosurgery cases. A recent 2021 manuscript directly compared SpeckleView with indocyanine green angiography demonstrating that the techniques provide different, yet complementary information about vessel perfusion.

Andrew Dunn was awarded the 2017 TAMEST Edith and Peter O’Donnell Award in Engineering for his pioneering work in developing laser speckle contrast imaging to visualize blood flow during surgery.

Peer-Reviewed Publications

The Dynamic Light team has 50+ peer-reviewed publications related to the development and application of SpeckleView® technology.

2021

Dynamics of isoflurane-induced vasodilation and blood flow of cerebral vasculature revealed by multi-exposure speckle imaging

C. T. Sullender, L. M. Richards, F. He, L. Luan, and A. K. Dunn, bioRxiv (2021)

Laser speckle contrast imaging for visualizing blood flow during cerebral aneurysm surgery: A comparison with indocyanine green angiography

D. R. Miller, R. Ashour, C. T. Sullender, and A. K. Dunn, medRxiv (2021)

2020

Effect of vascular structure on laser speckle contrast imaging

C. Z. Jafari, C. T. Sullender, D. R. Miller, S. A. Mihelic, and A. K. Dunn, Biomedical Optics Express (2020)

Peripheral electrical stimulation augments cerebral collateral circulation if performed within a critical time window

M.-C. Ding, A. Kundu, C. T. Sullender, and A. K. Dunn, bioRxiv (2020)

Multimodal mapping of neural activity and cerebral blood flow reveals long-lasting neurovascular dissociations after small-scale strokes

F. Hei, C. T. Sullender, H. Zhu, M. Williamson, X. Li, Z. Zhao, T. A. Jones, C. Xie, A. K. Dunn, and L. Luan, Science Advances (2020)

2019

Rehabilitative training interacts with ischemia-instigated spine dynamics to promote a lasting population of new synapses in peri-infarct motor cortex

T. A. Clark, C. T. Sullender, D. Jacob, Y. Zuo, A. K. Dunn, and T. A. Jones, Journal of Neuroscience (2019)

Artery targeted photothrombosis widens the vascular penumbra, instigates peri-infarct neovascularization and models forelimb impairments

T. A. Clark, C. T. Sullender, S. M. S. Kazmi, B. L. Speetles, M. R. Williamson, D. M. Palmberg, A. K. Dunn, and T. A. Jones, Scientific Reports (2019)

2018

Awake Mouse Imaging: From Two-Photon Microscopy to Blood Oxygen–Level Dependent Functional Magnetic Resonance Imaging

M. Desjardins, K. Kılıç, M. Thunemann, C. Mateo, D. Holland, C. G.L. Ferri, J. A Cremonesi, B. Li, Q. Cheng, K. L. Weldy, P. A. Saisan, D. Kleinfeld, T. Komiyama, T. T. Liu, R. Bussell, E. C. Wong, M. Scadeng, A. K. Dunn, D. A. Boas, S. Sakadžić, J. B. Mandeville, R. B. Buxton, A. M. Dale, and A. Devor, Biological Psychiatry: Cognitive Neuroscience and Neuroimaging (2018)

Extracellular Vimentin/VWF (von Willebrand Factor) Interaction Contributes to VWF String Formation and Stroke Pathology

T. A. Fasipe, S-H. Hong, Q. Da, C. Valladolid, M. T. Lahey, L. M. Richards, A. K. Dunn, M. A. Cruz, and S. P. Marrelli, Stroke (2018)

Imaging of cortical oxygen tension and blood flow following targeted photothrombotic stroke

C. T. Sullender, A. E. Mark, T. A. Clark, T. V. Esipova, S. A. Vinogradov, T. A. Jones, and A. K. Dunn, Neurophotonics (2018)

Nanoelectronics enabled chronic multimodal neural platform in a mouse ischemic model

L. Luan, C. T. Sullender, X. Li, Z. Zhao, H. Zhu, X. Wei, C. Xie, and A. K. Dunn, Journal of Neuroscience Methods (2018)

2017

Glioblastoma exosomes for therapeutic angiogenesis in peripheral ischemia

M. Desjardins, K. Kılıç, M. Thunemann, C. Mateo, D. Holland, C. G.L. Ferri, J. A Cremonesi, B. Li, Q. Cheng, K. L. Weldy, P. A. Saisan, D. Kleinfeld, T. A. Monteforte, B. Lam, M. B. Sherman, K. Henderson, A. D. Sligar, A. Spencer, B. Tang, A. K. Dunn, and A. B. Baker, Tissue Engineering Part A (2017)

Intraoperative multi-exposure speckle imaging of cerebral blood flow

L. M. Richards, S. M. S. Kazmi, K. E. Olin, J. S. Waldron, D. J. Fox, and A. K. Dunn, Journal of Cerebral Blood Flow & Metabolism (2017)

2016

Syndesome therapeutics for enhancing diabetic wound healing

S. Das, G. Singh, M. Majid, M. B. Sherman, S. Mukhopadhyay, C. S. Wright, P. E. Martin, A. K. Dunn, and A. B. Baker, Advanced Healthcare Materials (2016)

Glypican-1 nanoliposomes for potentiating growth factor activity in therapeutic angiogenesis

A. J. Monteforte, B. Lam, S. Das, S. Mukhopadhyay, C. S. Wright, P. E. Martin, A. K. Dunn, and A. B. Baker, Biomaterials (2016)

Syndecan-4 enhances therapeutic angiogenesis after hind limb ischemia in mice with type 2 diabetes

S. Das, A. J. Monteforte, G. Singh, M. Majid, M. B. Sherman, A. K. Dunn, and A. B. Baker, Advanced Healthcare Materials (2016)

Sensitivity of laser speckle contrast imaging to flow perturbations in the cortex

M. A. Davis, L. Gagnon, D. A. Boas, and A. K. Dunn, Biomedical Optics Express (2016)

2015

Evaluating multi-exposure speckle imaging estimates of absolute autocorrelation times

S. M. S. Kazmi, R. K. Wu, and A. K. Dunn, Optics Letters (2015)

Dynamic light scattering Monte Carlo: a method for simulating time-varying dynamics for ordered motion in heterogeneous media

M. A. Davis and A. K. Dunn, Optics Express (2015)

Flux or speed? Examining speckle contrast imaging of vascular flows

S. M. S. Kazmi, E. Faraji, M. A. Davis, Y. Huang, X. J. Zhang, and A. K. Dunn, Biomedical Optics Express (2015)

Expanding applications, accuracy, and interpretation of laser speckle contrast imaging of cerebral blood flow

S. M. S. Kazmi, L. M. Richards, C. J. Schrandt, M. A. Davis, and A. K. Dunn, Journal of Cerebral Blood Flow & Metabolism (2015)

Chronic monitoring of vascular progression after ischemic stroke using multiexposure speckle imaging and two-photon fluorescence microscopy.

C. J. Schrandt, S. M. S. Kazmi, T. A. Jones, and A. K. Dunn, Journal of Cerebral Blood Flow & Metabolism (2015)

2014

Intraoperative laser speckle contrast imaging with retrospective motion correction for quantitative assessment of cerebral blood flow

L. M. Richards, E. L. Towle, D. J. Fox, and A. K. Dunn, Neurophotonics (2014)

Imaging depth and multiple scattering in laser speckle contrast imaging

M. A. Davis, S. M. S. Kazmi, A. K. Dunn, Journal of Biomedical Optics (2014)

Optimization of camera exposure durations for multi-exposure speckle imaging of the microcirculation

S. M. S. Kazmi, S. Balial, A. K. Dunn, Biomedical Optics Express (2014)

Loss of Syndecan-1 Induces a Pro-Inflammatory Phenotype in Endothelial Cells with a Dysregulated Response to Atheroprotective Flow

P. L. Voyvodic, D. Min, R. Liu, E. Williams, V. Chitalia, A. K. Dunn, A. B. Baker, The Journal of Biological Chemistry (2014)

2013

Low-cost laser speckle contrast imaging of blood flow using a webcam

L. M. Richards, S. M. S. Kazmi, J. L. Davis, K. E. Olin, and A. K. Dunn, Biomedical Optics Express (2013)

Laser speckle contrast imaging of blood flow in rat retinas using an endoscope

A. Ponticorvo, D. Cardenas, A. K. Dunn, D. Ts’o, and T. Q. Duong, Journal of Biomedical Optics (2013)

Chronic imaging of cortical blood flow using Multi-Exposure Speckle Imaging

S. M. S. Kazmi, A. B. Parthasarthy, N. E. Song, T. A. Jones, and A. K. Dunn, Journal of Cerebral Blood Flow & Metabolism (2013)

2012

Comparison of indocyanine green angiography and laser speckle contrast imaging for the assessment of vasculature perfusion

E. L. Towle, L. M. Richards, S. M. S. Kazmi, D. J. Fox, and A. K. Dunn, Neurosurgery (2012)

Laser speckle contrast imaging of cerebral blood flow

A. K. Dunn, Annals of Biomedical Engineering (2012)

2011

Measurements and modeling of transient blood flow perturbations induced by brief somatosensory stimulation

K. Swanul, A. K. Dunn, T. Q. Duong, and D. Ress, The Open Neuroimaging Journal (2011)

Introduction: feature issue on In Vivo Microcirculation Imaging

A. K. Dunn, R. Leitgeb, R. K. Wang, and H. F. Zhang, Biomedical Optics Express (2011)

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl3 application: a novel model suitable for intravital microscopy and thrombolysis studies

H. Karatas, S. E. Erdener, Y. Gursoy-Ozdemir, G. Gurer, F. Soylemezoglu, A. K. Dunn, and T. Dalkara, Journal of Cerebral Blood Flow & Metabolism (2011)

2010

Laser speckle contrast imaging of cerebral blood flow in humans during neurosurgery: a pilot clinical study

A. B. Parthasarathy, E. L. Weber, L. M. Richards, D. J. Fox, and A. K. Dunn, Journal of Biomedical Optics (2010)

How to build a Laser Speckle Contrast Imaging (LSCI) system to monitor blood flow.

A. Ponticorvo and A. K. Dunn, Journal of Visualized Experiments (2010)

Quantitative imaging of ischemic stroke through thinned skull in mice with Multi Exposure Speckle Imaging

A. B. Parthasarathy, S. M. S. Kazmi, and A. K. Dunn, Biomedical Optics Express (2010)

Spreading depolarizations cycle around and enlarge focal ischaemic brain lesions.

H. Nakamura, A. J. Strong, C. Dohmen, O. W. Sakowitz, S. Vollmar, M. Sue, L. Kracht, P. Hashemi, R. Bhatia, T. Yoshimine, J. P. Dreier, A. K. Dunn, and R. Graf, Brain (2010)

Simultaneous imaging of oxygen tension and blood flow in animals using a digital micromirror device

A. Ponticorvo and A. K. Dunn, Optics Express (2010)

Laser speckle contrast imaging in biomedical optics

D. A. Boas and A. K. Dunn, Journal of Biomedical Optics (2010)

2009

Perfusion in hamster skin treated with glycerol

R. T. Zaman, A. B. Parthasarathy, G. Vargas, B. Chen, A. K. Dunn, H. G. Rylander, and A. J. Welch, Lasers in Surgery and Medicine (2009)

Sensitivity of neural-hemodynamic coupling to alterations in cerebral blood flow during hypercapnia.

T. J. Huppert, P. B. Jones, A. Devor, A. K. Dunn, I. C. Teng, A. M. Dale, and D. A. Boas, Journal of Biomedical Optics (2009)

2008

Stimulus-induced changes in blood flow and 2-deoxyglucose uptake dissociate in ipsilateral somatosensory cortex

A. Devor, E. Hillman, P. Tian, C. Waeber, I. C. Teng, L. Ruvinskaya, M. H. Shalinsky, H. Zhu, R. H. Haslinger, S. N. Narayanan, I. Ulbert, A. K. Dunn, E. H. Lo, B. R. Rosen, A. M. Dale, D. Kleinfeld, and D. A. Boas, The Journal of Neuroscience (2008)

Robust flow measurement with multi-exposure speckle imaging.

A. B. Parthasarathy, W. J. Tom, A. Gopal, X. Zhang, and A. K. Dunn, Optics Express (2008)

2007

Peri-infarct depolarizations lead to loss of perfusion in ischaemic gyrencephalic cerebral cortex

A. J. Strong, P. J. Anderson, H. R. Watts, D. J. Virley, A. Lloyd, E. A. Irving, T. Nagafuji, M. Ninomiya, H. Nakamura, A. K. Dunn, and R. Graf, Brain (2007)

2006

Vasoconstrictive neurovascular coupling during focal ischemic depolarizations

H. K. Shin, A. K. Dunn, P. B. Jones, D. A. Boas, M. A. Moskowitz, and C. Ayata, Journal of Cerebral Blood Flow & Metabolism (2006)

Evaluation of laser speckle flowmetry for imaging cortical perfusion in experimental stroke studies: quantitation of perfusion and detection of peri-infarct depolarisations

A. J. Strong, E. L. Bezzina, P. J. Anderson, M. G. Boutelle, S. E. Hopwood, and A. K. Dunn, Journal of Cerebral Blood Flow & Metabolism (2006)

2005

Spatial extent of oxygen metabolism and hemodynamic changes during functional activation of the rat somatosensory cortex

A. K. Dunn, A. Devor, A. M. Dale, and D. A. Boas, NeuroImage (2005)

Determination of optimal exposure time for imaging of blood flow changes with laser speckle contrast imaging

S. Yuan, A. Devor, D. A. Boas, and A. K. Dunn, Applied Optics (2005)

2004

Pronounced hypoperfusion during spreading depression in mouse cortex

C. Ayata, H. K. Shin, S. Salomone, Y. Ozdemir-Gursoy, D. A. Boas, A. K Dunn, and M. A. Moskowitz, Journal of Cerebral Blood Flow & Metabolism (2004)

Mouse model of microembolic stroke and reperfusion

D. N. Atochin, J. C. Murciano, Y. Gürsoy-Özdemir, T. Krasik, F. Noda, C. Ayata, A. K. Dunn, M. A. Moskowitz, P. L. Huang, and V. R. Muzykantov, Stroke (2004)

Laser speckle flowmetry for the study of cerebrovascular physiology in normal and ischemic mouse cortex

C. Ayata, A. K. Dunn, Y. Gursoy-OZdemir, Z. Huang, D. A. Boas, and M. A. Moskowitz, Journal of Cerebral Blood Flow & Metabolism (2004)

2003

Simultaneous imaging of total cerebral hemoglobin concentration, oxygenation, and blood flow during functional activation

A. K. Dunn, A. Devor, H. Bolay, M. L. Andermann, M. A. Moskowitz, A. M. Dale, and D. A. Boas, Optics Letters (2003)

2002

Intrinsic brain activity triggers trigeminal meningeal afferents in a migraine model

H. Bolay, U. Reuter, A. K. Dunn, Z. Huang, D. A. Boas, M. A. Moskowitz, Nature Medicine (2002)

2001

Dynamic imaging of cerebral blood flow using laser speckle

A. K. Dunn, H. Bolay, M. A. Moskowitz, and D. A. Boas, Journal of Cerebral Blood Flow & Metabolism (2001)